| 產(chǎn)品名稱 | STC-1 |
|---|---|
| 商品貨號(hào) | B167196 |
| Organism | Mus musculus, mouse |
| Tissue | intestine |
| Cell Type | intestinal neuroendocrine tumor cells |
| Product Format | frozen 1.0 mL |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | invasive small intestinal neuroendocrine carcinoma |
| Age | 10 to 13 weeks |
| Strain | C57B1/6J |
| Applications | This cell line may be a useful model for human neuroendocrine neoplasms of the gut, and useful tools for studying hormone secretin. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The STC-1 cell line was derived from the intestinal tumors of RIP1Tag2/Rip2pyST1 double transgenic mice. |
| Cellular Products | secretin |
| Comments | The STC-1 cell line was derived from the intestinal tumors of double transgenic mice. Transgenic mice harboring a hybrid gene linking the rat insulin promoter (RIP) to polyoma small T (PyST) antigen were mated with transgenic mice harboring rat insulin promoter (RIP) linked to SV40 early region (Tag) creating off-spring harboring both transgenes (double transgenics). These mice were found to have frequent intestinal tumors in addition to pancreatic Beta-cell tumors. Gene expression studies suggested that the intestinal and pancreatic tumors arose as separate entities. The STC-1cell line produces the hormone secretin. This cell line may be a useful model for human endocrine neoplasms of the gut. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Cells must be subcultured when they reach ~70% confluence, or else they start to come off the flask into suspension. Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Cells per Vial | 1.0 x 10^6 |
| Volume | 1.0 mL |
| Name of Depositor | Douglas Hanahan, PhD Cold Spring Harbor Laboratory |
| Year of Origin | June 1990 |
| References | Rindi G, et al. Development of Neuroendocrine Tumors in the Gastrointestinal Tract of Transgenic Mice. Am J Pathol 136(6): 1349-1363, 1990. PubMed: 2162628 Grant SGN, et al. Early Invasiveness Characterizes Metastatic Carcinoid Tumors in Transgenic Mice. Cancer Res 51: 4917-4923, 1991. PubMed: 1654206 |
| 序號(hào) | 文章標(biāo)題 | 操作 |
|---|---|---|
| 1 | ATCC細(xì)胞運(yùn)輸和處理 | 查看詳情 |
| 2 | 其他海外品牌代理 | 查看詳情 |
| 3 | ATCC代理 | 查看詳情 |
| 4 | 購(gòu)買須知 | 查看詳情 |
| 5 | 訂購(gòu)流程 | 查看詳情 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
