| Vector |
Construct size (kb): 8.710000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p3CLNeo
Intact vector size: 7.440
Type of vector: plasmid
Vector end: HindIII
Vector end: SalI
Cloning sites: HindIII SalI
Polylinker sites: HindIII SalI
Construction: p3CL, neoR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, G418
promoter: CMV
replicon: pMB1, SV40
terminator: bGH polyadenylation
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1DB*
Gene name: 5-hydroxytryptamine (serotonin) receptor 1D beta
Contains complete coding sequence?: Y
Tissue: placenta, male
Type of DNA: genomic
Insert 5' end: HindIII
Insert 3' end: SalI
Insert size (kb): 1.27
Cross references: DNA Seq. Acc.: L09732 Insert lengths(kb): 1.269999980926514 Tissue: placenta, male Gene product: 5-hydroxytryptamine (serotonin) receptor 1D beta [HTR1DB*] Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D beta |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.9, 1.8, 1.1, 0.7; HindIII/SalI--7.4, 1.3; PstI--6.5, 1.3, 0.9; PvuII--3.5, 2.3, 1.1, 0.78, 0.58, 0.44; SmaI--5.7, 3.0; EcoRI--6.0, 3.0; EcoRI/XhoI--6.0, 2.35, 0.65. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.30; NcoI--0.03; PstI--0.13; PvuII--0.80; SmaI--0.82. Includes 22 bp of 5' untranslated sequence, the complete coding region, and 64 bp of 3' untranslated sequence. The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - neoR - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - SalI - bGH polyadenylation - PvuI. |
| References |
Veldman SA, Bienkowski MJ. Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype. Mol. Pharmacol. 42: 439-444, 1992. PubMed: 1328844
Sarah A Veldman, personal communication
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