| 產品名稱 |
pV-mos |
| 商品貨號 |
B218292 |
| Designations |
pV-mos |
| GenBank Number |
J02266
|
| Species |
Moloney murine sarcoma virus, Moloney murine sarcoma virus |
| Depositors |
SA Aaronson, SR Tronick |
| Vector |
Construct size (kb): 5.199999809265137 |
| Insert |
DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: Moloney murine sarcoma viral (v-mos) oncogene homolog [MOS] Alleles: B2, B1 |
| Insert Size (kb) |
0.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
The BglI site on the insert end was replaced by a HindIII linker before cloning. The insert is the BglI/HindIII fragment from 4116 to 5021 on the Mo-MSV map. The insert contains KpnI, HpaI, SstII, PstI (4), HpaII (5), DdeI (3), and HinfI (4) sites. The insert excludes v-mos helper viral junctions. |
| Classification |
Retroviridae, Gammaretrovirus, Mammalian virus group |
| References |
Reddy EP, et al. Complete nucleotide sequence and organization of the Moloney murine sarcoma virus genome. Science 214: 445-450, 1981. PubMed: 6170110
Tronick SR, et al. Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome. Proc. Natl. Acad. Sci. USA 76: 6314-6318, 1979. PubMed: 392518
|
