ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 1.7, 1.0; EcoRI--4.4, 2.3; HindIII--4.6, 2.1; PvuII--3.7, 3.1; SalI--5.2, 1.6.
The insert includes the following restriction sites (kb from the 5' end): BamHI--0.027, 1.629; HindIII--0.231; ScaI--1.022; SalI--1.403.
Contains the complete coding sequence but lacks some of the 5' untranslated region. The coding sequences extend from nt 154 to 1709 of the published sequence.
Constructed from G5A-IY and ID9bb by taking advantage of the unique ScaI site in the region of overlap.
References
Zimran A, et al. Linkage of the PvuII polymorphism with the common Jewish mutation for gaucher disease. Am. J. Hum. Genet. 46: 902-905, 1990. PubMed: 1971142
Dahl N, et al. Gaucher disease type III (Norrbottnian type) is caused by a single mutation in exon 10 of the glucocerebrosidase gene. Am. J. Hum. Genet. 47: 275-278, 1990. PubMed: 2378352
Sorge J, et al. Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA. Proc. Natl. Acad. Sci. USA 82: 7289-7293, 1985. PubMed: 3864160
Sorge J, et al. Correction. Proc. Natl. Acad. Sci. USA 83: 3567, 1986.
Sorge JA, et al. The human glucocerebrosidase gene has two funtional ATC initiator codons. Am. J. Hum. Genet. 41: 1016-1024, 1987. PubMed: 3687939
