| 產品名稱 |
pEGsacIIRMC5 [NEB673] |
| 商品貨號 |
B220467 |
| Designations |
pEGsacIIRMC5 [NEB673] |
| Species |
Streptomyces achromogenes Okami and Umezawa |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications |
produces protein modification methylase SacII produces protein restriction endonuclease SacII |
| Insert |
DNA: genomic Insert lengths(kb): 3.650000095367432 Gene product: restriction endonuclease SacII [hsdM] Target Gene: modification methylase SacII, restriction endonuclease SacII |
| Insert Size (kb) |
3.650 |
| Media |
ATCC® Medium 1339: R2 YE medium
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Freeze dried S. lividans containing the plasmid |
| Comments |
The insert contains the following restriction sites (approximate kb from the 5' end): ApaI--0.6, 2.1; BamHI--0.9, 3.0; BglII--2.2; NcoI--1.1, 1.8, 2.8; PstI--2.5. The methylase gene is contained in the 2.5 kb HindIII/PstI fragment. Verified to be viable in R2 YE medium + 50 ug/ml thiostrepton. |
| References |
Guthrie EP, Meda MM. Method for producing and cloning SacII restriction endonuclease and methylase. US Patent 5,288,696 dated Feb 22 1994
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
