| 產品名稱 |
pRY252 |
| 商品貨號 |
B220731 |
| Designations |
pRY252 |
| Depositors |
RR Yocum |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli YMC9 |
| Vector Information |
Size (kb): 9.3000001907348630 Vector: pRY252 (plasmid) Promoters: Promoter CYC1 Construction: pBR322 Marker(s):ampR G418R URA3 Construct size (kb): 9.300000190734863 Features: marker(s): G418R
marker(s): URA3
marker(s): ampR
promoter: CYC1
replicon: 2 micron
replicon: pMB1 |
| Applications |
YE-type (episomal) shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0, 2.3; SalI--9.3; PvuII--uncut; XbaI--9.3; XhoI--8.9, 0.4. YE-type shuttle vector conferring G418 resistance under control of the CYC1 promoter. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995
|
| Shipping Information |
Distributed: freeze-dried |
