| 產(chǎn)品名稱 |
5B1-4 |
| 商品貨號 |
B220841 |
| Designations |
5B1-4 |
| GenBank Number |
L10338
|
| Species |
Homo sapiens, human |
| Depositors |
JF Gusella |
| Vector |
Construct size (kb): 4.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN1B
Gene name: sodium channel, voltage-gated, type I, beta polypeptide
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.1
Tissue: frontal cortex
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.404
Cross references: DNA Seq. Acc.: L10338
Nucleotides 1-1404 of the insert correspond to
nucleotides 1-1404 of L10338 with 100% identity. Insert lengths(kb): 1.404000043869019 Tissue: frontal cortex Gene product: sodium channel, voltage-gated, type I, beta polypeptide(sodium channel, voltage-gated, beta 1 polypeptide) [SCN1B] |
| Insert Size (kb) |
1.404 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3, 0.72 (doublet); EcoRI--2.9, 1.5; PstI--3.9, 0.45; PvuII--2.5, 1.25, 0.62; SstI--4.4. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.28, 0.95; NdeI--0.61; NcoI--0.10; PstI--0.40; PvuII--0.33; SmaI--0.06. Detects a 1.5 kb transcript in several brain regions and skeletal muscle. |
| References |
McClatchey AI, et al. The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain. Hum. Mol. Genet. 2: 745-749, 1993. PubMed: 8394762
|
