| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.3, 14.0, 9.2, 4.0, 2.8; EcoRI/HindIII--23.0, 9.0, 5.5, 5.4, 4.3, 2.5, 2.2, 1.7. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): CTGCAGCCCACTTCCAGTTCCCTTC and TTCTTCACTGTGAGTCCTTTCATGGC. The predicted product size is (bp): 219. The observed product size is (bp): 219. |
