| 產品名稱 |
pCM56 |
| 商品貨號 |
B221251 |
| Designations |
pCM56 |
| GenBank Number |
X02981
|
| Species |
Escherichia coli bacteriophage T3 |
| Depositors |
Univ. Med. & Dent. New Jersey, LN Stephenson, Univ. Med. & Dent. New Jersey |
| Applications |
produces protein RNA polymerase |
| Vector |
Construct size (kb): 8.48
Name of vector: pCM53
Intact vector size: 5.758
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: pAR1234 (deletion of the 346 bp HindIII/BamHI fragment
following the lacUV5 promoter)
Host range: Escherichia coli
Features (with orientation and position when available):
restriction site: BamHI, 3-3
replicon: pMB1, ← 2128-2128
marker(s): ampR, ← 2923-3783
repressor gene: lacI, → 4106-5180
promoter for expression: lacUV5, → 5273-5273 |
| Insert |
Genome: bacteriophage T3
Gene symbol: gene 1
Gene name: RNA polymerase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: Modification: BamHI linker
Insert end: Modification: BamHI linker
Insert size (kb): 2.712
Cross references: DNA Seq. Acc.: X02981
Nucleotides 1-2712 of the insert correspond to
nucleotides 96-2807 of X02981.
Insert lengths(kb): 2.71
Gene product: RNA polymerase [gene 1]
Target Gene: RNA polymerase |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Frozen glycerol stock of E. coli containing the plasmid. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.55, 2.7; HindIII--9.4; NdeI--6.55, 2.7. Insert contains the following restriction sites (approximate kb from the 5' end): HincII--0.3, 1.0, 1.7; NdeI--1.9; StuI--0.4. Cloned from a 5.9 kb MboI fragment of bacteriophage T3. The fragment was digested with MstI and AflII and treated with Bal31 to remove promoter sequences. Expression in this construct is regulated by the lacUV5 promoter and inducible by IPTG. |
| References |
McAllister WT. Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids. US Patent 5,037,745 dated Aug 6 1991
Morris CE, et al. Cloning and expression of the bacteriophage T3 RNA polymerase gene. Gene 41: 193-200, 1986. PubMed: 3011596
McGraw NJ, et al. Sequence and analysis of the gene for bacteriophage T3 RNA polymerase. Nucleic Acids Res. 13: 6753-6766, 1985. PubMed: 3903658
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
