Restriction digests of the clone give the following sizes (kb): HindIII--4.0, 2.8; BglI/XhoI--4.5, 2.3; SphI--6.8. SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein. Vector for use the the SOS Recruitment System (SRS). It encodes the v-Src myristoylation signal and can be used to construct expression libraries to be screened for interacting proteins. |
