| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 0.514, 4.285, 0.914, 0.704, 7.887, 3.152, 2.060, 0.636, 1.632, 1.520, 0.894, 0.590, 0.484, 0.466, 4.040, 0.549. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.45, 0.51, 0.67, 0.89, 1.5, 1.6, 2.1, 3.3, 4.2, 4.4, 8.2. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70145, 70522, 70840. |
