| 產品名稱 |
AA5 |
| 商品貨號 |
B221709 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The AA5 antibody restores strong adhesion to activin-treated blastomeres. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with purified CEC1-5 (soluble C-cadherin ectodomain) protein. Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against C-cadherin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against C-cadherin |
| Comments |
Animals were immunized with purified CEC1-5 (soluble C-cadherin ectodomain) protein. Spleen cells were fused with Sp2/0 mouse myeloma cells. C-cadherin is the major cadherin expressed in the blastula and early gastrula and is largely responsible for holding cells in the embryo together. The AA5 antibody is the first antibody that can activate cadherin adhesive function. The AA5 antibody restores strong adhesion to activin-treated blastomeres. The C-cadherin-activating antibody strongly inhibits the elongation of animal caps, in response to activin, without affecting mesodermal gene expression. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 5 X 10(4) and 8 X 10(5) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse kappa |
| Name of Depositor |
BM Gumbiner |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Zhong Y, et al. Analysis of C-cadherin regulation during tissue morphogenesis with an activating antibody. J. Cell Biol. 144: 351-359, 1999. PubMed: 9922460
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