| Vector Information |
Size (kb): 4.9000000953674320 DESCRIPTION OF VECTOR:
Intact vector size: 4.900
Type of vector: plasmid
Cloning sites: SmaI
Polylinker sites:
Construction: P15A ori, sacB, RP4, gtmR
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
replicon: oriV, p15A
other: traJ, <-
marker(s): sacB, ->
replicon: oriT
marker(s): gtmR, <- Vector: pJQ200 (plasmid) Construction: P15A ori, sacB, RP4, gtmR Marker(s):sacB,gtmR Construct size (kb): 4.900000095367432 Features: marker(s): gtmR
marker(s): sacB
other: traJ
replicon: oriT
replicon: oriV, p15A |
| Comments |
Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2; HindIII--5.2. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g. as a BamHI cassette). When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. The order of the major features of the plasmid is: gtmR - SmaI - P15A - traJ - oriT - sacB. |
