| 產品名稱 |
Adeno-associated virus 3 |
| 商品貨號 |
B222891 |
| Classification |
Parvoviridae, Dependovirus |
| Agent |
Adeno-associated virus 3 |
| Strain |
H |
| Common Name |
AAV-3H |
| Applications |
Cross-reacts with AAV-2; no hemagglutinin detected. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
?-70°C or colder |
| Comments |
Requires co-infection with helper adenovirus Adenovirus type 2 (ATCC VR-846) for replication. Helper virus may be inactivated after harvest in water bath at 56°C for 30 minutes. Cross-reacts with AAV-2; no hemagglutinin detected. |
| Effect on Host |
AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing. |
| Recommended Host |
Production Host: HEK-293 (ATCC CRL-1573). Production Host: ?HEK-293 (ATCC? CRL-1573™) Alternate Hosts: HEK cells, KB cells (ATCC CCL-17) and HeLa cells (ATCC CCL-2) |
| Growth Conditions |
Temperature: 37°C Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with helper adenovirus and a small volume (e.g. 1 mL per 25 cm2) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 2-3 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor |
MD Hoggan |
| Chain of Custody |
Deposited as Adeno-associated virus type 3 |
| Source |
Adenovirus 7, SV-40 hybrid E 46 plus strain infected HEK cells |
| References |
Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666
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