Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8, 0.4, 0.2; PstI--4.8, 0.6; BamHI--5.4; BamHI/HindIII--3.0, 1.7, 0.7. Insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.68, 0.75; PstI--0.74; PvuII--0.74; SacI--0.03, 0.08. Insert contains 164 bp 5' untranslated sequence and about 1.6 kb of 3' untranslated sequence. Detects a 4.5 kb mRNA transcript. |
