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Construct size (kb): 4.639999866485596

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCRII
Intact vector size: 3.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV
BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, kanR
promoter: lac, SP6, T7
replicon: pMB1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBP4
Genomic copy number: unique
Gene name: retinol-binding protein 4, interstitial
Contains complete coding sequence?: Y
Chromosome: 10; Localization: 10 q23-q24
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.736
Cross references: DNA Seq. Acc.: X00129

Insert lengths(kb): 0.7360000014305115

Tissue: liver

Gene product: retinol-binding protein 4, interstitial [RBP4]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 0.74; PstI--3.3, 1.2, 0.29; SalI--4.8; NcoI--3.0, 1.75; AvaI--4.1, 0.40, 0.30.

Constructed by amplification using primers annealing between nt 46 and 756 of the published sequence. Alignment of this sequence with the previous requires 3 gaps and 5 substitutions, and the reading frame is not maintained.

The insert contains the following restriction sites (bp from the 5' end): AvaI--163, 481; NcoI--146; NotI--61; PstI--178; SalI--387; SmaI--481.

Contains the complete coding sequence (nt 52-648 of GenBank accession X00129).

Transcription from the T7 promoter gives the sense strand.