| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7; HindIII--6.6; PstI--6.6; BamHI--6.6; PvuII--4.4, 3.2. The insert contains HaeIII, SacI (2), MboII, AccI, and HphI sites. The insert includes the complete exon for the constant region plus 5' and 3' flanking sequences. The polymorphic SacI site is located 3' to the SacI site in the coding sequence of the kappa constant region. Enzyme(s) not detecting polymorphism: ApaI, AvaII, BamHI, BclI, BglII, BstEII, EcoRI, EcoRV, HincII, HindIII, HinfI, KpnI, MboI, MspI, PstI, PvuII, TaqI, XbaI (tested on 7 unrelated Caucasians). |
| References |
Field LL, et al. SacI RFLP recognized by a human immunoglobulin kappa light chain constant region probe. Nucleic Acids Res. 15: 3942, 1987. PubMed: 2884643
Hieter PA, et al. Evolution of human immunoglobulin kappa J region genes. J. Biol. Chem. 257: 1516-1522, 1982. PubMed: 6276389
Weir L, Leder P. Structure and expression of a human subgroup II immunoglobulin kappa gene. Nucleic Acids Res. 14: 3957-3970, 1986. PubMed: 3086847
Hieter PA, et al. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell 22: 197-207, 1980. PubMed: 6775818
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