Gene product: T cell receptor, gamma, variable region V10 [TCRGV10]
Insert Size (kb)
0.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.0; EcoRI/HindIII--2.6, 0.4; EcoRI--3.0; XbaI--3.0, PstI--3.0.
TCRGV10 is the unique member of the V gamma III gene subgroup.
The 5' end of the gene is available as pV10PR (ATCC 59598, 59599).
Subcloned from lambdaJM15. Contains the 3' region of TCRGV10. The BamHI site was not reconstituted during cloning.
References
Huck S, et al. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 7: 719-726, 1988. PubMed: 2969332
Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744
Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738
Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713
