| Vector |
Construct size (kb): 6.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: APXL
Gene name: apical protein, Xenopus laevis-like
Contains complete coding sequence?: N
Tissue: retina, adult
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.647
Cross references: DNA Seq. Acc.: X83543
Nucleotides 1-3637 of the insert correspond to
nucleotides 3808-7445 of X83543. Insert lengths(kb): 3.647000074386597 Tissue: retina, adult Gene product: apical protein, Xenopus laevis-like [APXL] Target Gene: apical protein, Xenopus laevis-like |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.0; BamHI--6.3, 0.4; HindIII-- 6.0, 0.7. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.35; BglI--0.05, 0.15, 0.55, 2.85, 2.9; HindIII--3.05; NcoI--2.15; SacI--0.65, 1.0, 1.25; SmaI--0.85; PstI--0.6, 0.7; PvuII--1.15, 3.05, 3.35, 3.5; XhoI--3.3. The insert contains a portion of the coding sequence and 2509 bp of 3' untranslated sequence, overlapping AR24 (ATCC 99512) at the 5' end. The coding region of GenBank X83543 corresponds to nt 91-4941. |
