| 產品名稱 |
pHEBo1 [pHEBo] |
| 商品貨號 |
B227154 |
| Designations |
pHEBo1 [pHEBo] |
| Depositors |
Wisconsin Alumni Res. Fndn., HW Bremer, Wisconsin Alumni Res. Fndn. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli |
| Vector Information |
Size (kb): 6.9000000953674320 Vector: pHEBo1 (plasmid) Promoters: Promoter HSV TK Construction: EBV, HSV-1, pBR322 Marker(s):ampR,hygR Construct size (kb): 6.900000095367432 Features: marker(s): ampR, hygR
promoter: HSV TK
replicon: pMB1, EBV oriP |
| Applications |
shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.9; ClaI--6.9; EcoRI--4.4, 2.3; EcoRV--4.6, 2.4; HindIII--6.9; SalI--6.9. A map is provided in this reference. Shuttle vector that can replicate and be selected in Escherichia coli and mammalian cells. The order of the major features in this plasmid is: EcoRI - ClaI - HindIII - BamHI - SalI - oriP - HSV-1 tk polyadenylation - <- hygR - HSV-1 tk promoter - < - ampR. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Sugden WM. Recombinant vector and eukaryotic host transformed thereby. US Patent 4,686,186 dated Aug 11 1987
. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1989.
|
| Shipped |
freeze-dried |
