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Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn)
Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn)
規格:
貨期:
編號:B227285
品牌:Mingzhoubio

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    • 產品名稱 Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn)
      商品貨號 B227285
      Organism Homo sapiens, human
      Tissue Skin
      Cell Type Fibroblast
      Morphology Spindle-shaped; cells are bipolar and refractile.
      Growth Properties Adherent
      Biosafety Level 1

      Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
      Disease Normal
      Age Neonatal
      Gender Male
      Ethnicity Batch-specific
      Applications
      Response to pathogens, skin aging, wound healing, gene delivery, skin diseases (e.g., scleroderma)
      Product Format frozen 1 mL
      Storage Conditions -130°C or below
      Comments
      Serum-free medium supports excellent growth curves and normal morphology, as well as serum-free (not animal-free) experimental conditions. The presence of 2% fetal bovine serum in the Fibroblast Growth Kit-Low serum (ATCC PCS-201-041) supports more prolific growth.
      Complete Growth Medium
      1. Obtain one growth kit from the freezer; make sure that the caps of all containers are tight.
      2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates prior to adding to the basal medium.
      3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
      4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
      5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
      6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
      7. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.

       

      Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown. 

        Component

         

         
        Subculturing

        Volume

         

         
        Volume

        Final Concentration

         

         
        Sterility Tests

        L-glutamine

         

         
        Viral Testing

        18.75 mL

         

         
        Viability

        7.5 mM

         

         
        Population Doubling Capacity

        Hydrocortisone Hemisuccinate

         

         
        C of A

        0.5 mL

         

         
        梅經理 17280875617 1438578920
        胡經理 13345964880 2438244627
        周經理 17757487661 1296385441
        于經理 18067160830 2088210172
        沈經理 19548299266 2662369050
        李經理 13626845108 972239479
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