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Size (kb): 5.78

Vector: pXC46 (plasmid)

Promoters: Promoter for expression lambda PL

Construction: pXC24, oligo cassette

Marker(s):ampR

Construct size (kb): 5.77

Features: initiation codon: ATG
marker(s): ampR
marker(s): tetR
promoter for expression: lambda PL
replicon: ROP copy number control
replicon: pMB1
restriction site: 3' sites HindIII...BamHI
restriction site: 5' SwaI...NsiI/NdeI sites
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: ATTTAA
transcription terminator: partially deleted lambda terminator
coding sequence: 14-3-3

expression vector

in another host, produces protein

vector useful for cloning PCR products

Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.

The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.

One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.

The insert is expressed as a Met-His fusion protein. Sequences cloned into the NsiI/NdeI site are expressed as a Met-His fusion protein, while sequences cloned into the SwaI site can be expressed as an unfused protein (must provide ATG in cloned insert).

Temperature: 37.0°C