| 產品名稱 |
pcDV1 |
| 商品貨號 |
B230106 |
| Designations |
pcDV1 |
| Depositors |
H Okayama |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 3.0000000000000000 Vector: pcDV1 (plasmid) Promoters: Promoter none Construction: pBR322, SV40 Marker(s):ampR Construct size (kb): 3.0 Features: marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
enhancer: none |
| Applications |
expression vector vector for constructing cDNA libraries |
| Comments |
pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to construct a cDNA library. The SV40 segment contains KpnI and EcoRI sites for the preparation of vector-primer containing a poly(dT) tail at one end and the polyadenylation sequence from the late region of the virus. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662
|
| Related Products |
component of:ATCC 77361 |
| Shipping Information |
Distributed: freeze-dried |
