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Size (kb): 6.3710000000000000

Vector: pZJMalpha (plasmid)

Type: expression

Construction: A 656bp fragment of tubulin is cloned into XhoI/HindIII site.

Construct size (kb): 6.4

Features: marker(s): ampR, BleoR
operator: Tet
promoter: T7
replicon: E. coli Ori
MCS: XhoI, XbaI, NdeI, HpaI, HindIII, SfiI
terminator: T7
transcription terminator: Actin3' UTR, Aldolase 3' UTR

vector for studying DNA-protein interactions tubulin, alpha

PCR-amplified gene fragments can be ligated in a single step between two opposing T7 promoters. This vector can integrate into a nontranscribed region of the Trypanosoma brucei genome and establish a stable cell line. Tetracycline induction leads to the production of a dsRNA with concomitant degradation of the target mRNA.

Temperature: 37.0°C