| 產(chǎn)品名稱 | PC-12 Adh |
|---|---|
| 商品貨號 | B234792 |
| Organism | Rattus norvegicus, rat |
| Tissue | adrenal gland |
| Product Format | frozen |
| Morphology | polygonal |
| Culture Properties | adherent, small clusters |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | pheochromocytoma |
| Gender | male |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | 40 chromosomes; 38 autosomes plus XY
Ref  Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Images |  |
| Derivation | The PC-12 cell line was derived from a transplantable rat pheochromocytoma. |
| Clinical Data | male |
| Genes Expressed | catecholamines; dopamine; norepinephrine
RefGreene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Cellular Products | catecholamines; dopamine; norepinephrine
Ref Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Tumorigenic | Yes |
| Effects | Yes, in New England Deaconess Hospital strain rats |
| Comments | The cells do not synthesize epinephrine. This adherent variant (PC-12 Adh) has been adapted to Corning CellBIND® flasks to improve cell attachment.
ATCC has not tested CRL-1721.1 cells for differentiation potential in response to NGF treatment. Please refer to the ATCC CRL-1721 cell line which responds reversibly to NGF by induction of the neuronal phenotype. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
|
| Subculturing | Use Corning CellBIND flasks (Corning catalog #3289 through 3293) Volumes used for this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time | 48 hrs |
| Name of Depositor | B Patterson |
| Deposited As | Rattus sp. |
| References | Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712 Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
