The insert contains the following restriction sites (kb): BamHI--4.449, 3.417; EcoRI--5.180, 2.686; KpnI--6.365, 1.501; SalI--7.866; SfiI--7.866; XhoI--uncut Plasmid size: 7.866 kb [2.686 kb (pUC19 vector) + 5.180 kb (SV40-777-1 insert)] Plasmid copy#: High-copy number (pUC19 has a ColEI ori) Viable virus clone. Verified by lipofection of EcoRI-linearized plasmid DNA into ATCC CV-1 cells (passage# 40) by J. Lednicky (lipofection date: 12 Oct. 2000). This construct is an archetypal-regulatory region variant of SV40-777. |
