| 產(chǎn)品名稱 | Influenza A virus (H1N1) |
|---|---|
| 商品貨號 | B235498 |
| Classification | Orthomyxoviridae, Influenzavirus A |
| Deposited As | Influenza A virus |
| Agent | Influenza A virus (H1N1) |
| Strain | A/PR/8/34 |
| Applications | Influenza Research |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | VR-1469® is a TC-adapted item derived from virus passaged in eggs (ATCC® VR-95™). |
| Effect on Host | CPE, rounding, degeneration, sloughing |
| Recommended Host | MDCK cells (ATCC® CCL-34™) |
| Growth Conditions | Temperature: 35°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | ATCC®, M Coleman, Centers for Disease Control and Prevention |
| Special Collection | NCRR Contract |
| Source | Patient in Puerto Rico, 1934 |
| References | Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
