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Construct size (kb): 8.90000057220459

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRL
Genomic copy number: unique
Gene name: glucocorticoid receptor
Contains complete coding sequence?: U
Chromosome: 5; Localization: 5 q31-q32
Tissue: leukocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 4.5
Cross references: DNA Seq. Acc.: M73050

Insert lengths(kb): 4.5

Tissue: leukocyte

Gene product: glucocorticoid receptor [GRL]

Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 4.5; HindIII--9.3; PstI--6.2, 3.0; SacI--8.6, 0.52; XbaI--9.3.

The insert contains the following restriction sites (approximate kb from the 5' end: SmaI--1.8, 3.4; SacI--2.1, 2.6; PstI--2.4; NcoI--2.8, XbaI--4.1.

Contains the promoter region, exon 1 and part of intron 1.

Subcloned from lambdaGHGR-5. ATCC 80008/9 and ATCC 80010/11 are contiguous in the genome.