| 產品名稱 | sNF02.2 |
|---|---|
| 商品貨號 | B238713 |
| Organism | Homo sapiens, human |
| Tissue | derived from metastatic site: lung |
| Cell Type | Schwann Cell |
| Product Format | frozen |
| Morphology | Schwann cell-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | neurofibromatosis type 1 (NF1) |
| Age | 35 year old |
| Gender | male |
| Storage Conditions | Liquid nitrogen vapor phase |
| Images |  |
| Derivation | The sNF02.2 was derived from a lung metastasis diagnosed by histopathology as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin protein. |
| Clinical Data | 35 years
male |
| Comments | Western immunoblotting showed apparently full-length neurofibromin protein. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 twice weekly is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Culture medium,90%; DMSO,10%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: 5% CO2 in air recommended Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 8,12 vWA: 16 |
| Population Doubling Time | about 26 hours |
| Name of Depositor | DF Muir, MR Wallace |
| Deposited As | Homo sapiens |
| Passage History | -The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. - |
| Year of Origin | 2002 |
| References | Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937 Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
