| 產品名稱 |
pTHH37 [RW-23] |
| 商品貨號 |
B239590 |
| Designations |
pTHH37 [RW-23] |
| Species |
Homo sapiens, human |
| Depositors |
RL White, Y Nakamura |
| Applications |
RFLPs observed under normal hybridization stringency; washes 0.1X SSC, 0.1% SDS, 65 C. |
| Vector |
Construct size (kb): 5.099999904632568 |
| Insert |
DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA Segment, single copy [D14S16] Alleles: A1, A2, A3 |
| Insert Size (kb) |
2.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; HindIII--4.2, 0.98; BamHI--5.0; AvaI--3.1, 2.0. PstI detects a 3 allele polymorphism with bands between 2-3 kb. MspI and TaqI detect the same polymorphism. Codominant segregation has been shown in 60 families. RFLPs observed under normal hybridization stringency; washes 0.1X SSC, 0.1% SDS, 65 C. Enzyme(s) not detecting polymorphism: RsaI. |
| References |
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH37) on chromosome 14 [D14S16]. Nucleic Acids Res. 16: 3593, 1988. PubMed: 2897662
Peter J O'Connell, personal communication
|
