| 產(chǎn)品名稱(chēng) |
pAMY8 |
| 商品貨號(hào) |
B239740 |
| Designations |
pAMY8 |
| GenBank Number |
X03236
|
| Species |
Bacillus licheniformis (Weigmann) Chester |
| Depositors |
T Thiel |
| Applications |
contains sequence amylase, alpha 1A; salivary Amylase, salivary, alpha-1A |
| Vector |
Construct size (kb): 6.300000190734863 |
| Insert |
DNA: genomic Insert lengths(kb): 3.299999952316284 Tissue: Bacillus licheniformis Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) [amyE] Target Gene: amylase, alpha 1A; salivary |
| Insert Size (kb) |
3.300 |
| Media |
ATCC Medium 1065 (see below) plus kanamycin (50 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): ClaI--4.4, 2.0; EcoRI--6.5; HindIII--3.2 (doublet). Alpha amylase expression expressed in Escherichia coli, NPT encodes kanamycin resistance. Insert includes more than one sequenced gene. |
| References |
Elhai J, Wolk CP. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138, 1988. PubMed: 2851487
|
