| 產(chǎn)品名稱 | 20B8 |
|---|---|
| 商品貨號(hào) | B239841 |
| Organism | Homo sapiens, human |
| Tissue | embryonic kidney |
| Cell Type | epithelial; somatic hybrid transfected with plasmid pSV2ne |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Burkitt's lymphoma |
| Applications | The 20B8 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pCIS25DTR expression vector coding for a B-domain deleted (BDD) FVIII. This cell line can be used for high productivity of B-domain deleted Factor VIII. |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images |  |
| Derivation | The 20B8 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pCIS25DTR expression vector coding for a B-domain deleted (BDD) FVIII. This cell line can be used for high productivity of B-domain deleted Factor VIII. |
| Comments | The 20B8 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pCIS25DTR expression vector coding for a B-domain deleted (BDD) FVIII. This cell line can be used for high productivity of B-domain deleted Factor VIII. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,11,12 D13S317: 12,14 D16S539: 9 D5S818: 8,9,12 D7S820: 10,11 THO1: 7,9,9.3 TPOX: 6,11 vWA: 15,19 |
| Name of Depositor | Bayer Corporation |
| U.S. Patent Number | |
| References | Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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