| 產(chǎn)品名稱(chēng) |
pVH17 |
| 商品貨號(hào) |
B241693 |
| Designations |
pVH17 |
| GenBank Number |
X03224
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
C Wong |
| Applications |
produces protein 2-deoxyribose-5-phosphate aldolase |
| Vector |
Construct size (kb): 5.069999217987061 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: AvaI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: deoC
Gene name: 2-deoxyribose-5-phosphate aldolase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: EcoRI
Insert 3' end: AvaI
Insert size (kb): 2.13
Cross references: DNA Seq. Acc.: X03224 Insert lengths(kb): 2.130000114440918 Gene product: 2-deoxyribose-5-phosphate aldolase [deoC] Target Gene: 2-deoxyribose-5-phosphate aldolase |
| Insert Size (kb) |
2.130 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 0.5; BglII--5.0; EcoRI/AvaI--3.0, 1.6, 0.5. The 0.5 kb EcoRI and the 1.6 kb AvaI/EcoRI fragment are from the insert. The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.46, 0.53; EcoRI--0.48; BglII--0.59. Contains the two tandem promoter/operator regions for the deo operon, the complete coding sequence for deoC, and 53 bp of deoA. The insert includes approximately 350 bp 5' sequence not in the GenBank record. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Valentin-Hansen P, et al. The structure of tandem regulatory regions in the deo operon of Escherichia coli K12. EMBO J. 1: 317-322, 1982.
Chen L, et al. Deoxyribose-5-phosphate aldolase as a catalyst in asymmetric aldol condensation. J. Am. Chem. Soc. 114: 741-748, 1992.
Valentin-Hansen P, et al. The primary structure of Escherichia coli K12 2-deoxyribose 5-phosphate aldolase. Nucleotide sequence of the deoC gene and the amino acid sequence of the enzyme. Eur. J. Biochem. 125: 561-566, 1982. PubMed: 6749498
|
